diff --git a/packages/react-native/Libraries/Components/ScrollView/__tests__/ScrollView-viewCulling-itest.js b/packages/react-native/Libraries/Components/ScrollView/__tests__/ScrollView-viewCulling-itest.js
index 0eb07f196e5..b0daeb7ec4d 100644
--- a/packages/react-native/Libraries/Components/ScrollView/__tests__/ScrollView-viewCulling-itest.js
+++ b/packages/react-native/Libraries/Components/ScrollView/__tests__/ScrollView-viewCulling-itest.js
@@ -840,3 +840,112 @@ test('culling when ScrollView parent has transform', () => {
     'Insert {type: "View", parentNativeID: (root), index: 0, nativeID: (N/A)}',
   ]);
 });
+
+test('view flattening with culling', () => {
+  const root = Fantom.createRoot({viewportWidth: 100, viewportHeight: 100});
+  let maybeNode;
+
+  Fantom.runTask(() => {
+    root.render(
+      <ScrollView
+        style={{height: 100, width: 100}}
+        ref={node => {
+          maybeNode = node;
+        }}>
+        <View
+          style={{
+            marginTop: 150,
+          }}>
+          <View
+            nativeID={'child'}
+            style={{height: 10, width: 10, backgroundColor: 'red'}}
+          />
+        </View>
+      </ScrollView>,
+    );
+  });
+
+  expect(root.takeMountingManagerLogs()).toEqual([
+    'Update {type: "RootView", nativeID: (root)}',
+    'Create {type: "ScrollView", nativeID: (N/A)}',
+    'Create {type: "View", nativeID: (N/A)}',
+    'Insert {type: "View", parentNativeID: (N/A), index: 0, nativeID: (N/A)}',
+    'Insert {type: "ScrollView", parentNativeID: (root), index: 0, nativeID: (N/A)}',
+  ]);
+
+  const element = ensureInstance(maybeNode, ReactNativeElement);
+
+  Fantom.runOnUIThread(() => {
+    Fantom.scrollTo(element, {
+      x: 0,
+      y: 60,
+    });
+  });
+
+  Fantom.runWorkLoop();
+
+  expect(root.takeMountingManagerLogs()).toEqual([
+    'Update {type: "ScrollView", nativeID: (N/A)}',
+    'Create {type: "View", nativeID: "child"}',
+    'Insert {type: "View", parentNativeID: (N/A), index: 0, nativeID: "child"}',
+  ]);
+
+  // force view to be unflattened.
+  Fantom.runTask(() => {
+    root.render(
+      <ScrollView
+        style={{height: 100, width: 100}}
+        ref={node => {
+          maybeNode = node;
+        }}>
+        <View
+          style={{
+            marginTop: 150,
+            opacity: 0, // force view to be unflattened
+          }}>
+          <View
+            nativeID={'child'}
+            style={{height: 10, width: 10, backgroundColor: 'red'}}
+          />
+        </View>
+      </ScrollView>,
+    );
+  });
+
+  expect(root.takeMountingManagerLogs()).toEqual([
+    'Update {type: "View", nativeID: "child"}',
+    'Remove {type: "View", parentNativeID: (N/A), index: 0, nativeID: "child"}',
+    'Create {type: "View", nativeID: (N/A)}',
+    'Insert {type: "View", parentNativeID: (N/A), index: 0, nativeID: (N/A)}',
+    'Insert {type: "View", parentNativeID: (N/A), index: 0, nativeID: "child"}',
+  ]);
+
+  // force view to be flattened.
+  Fantom.runTask(() => {
+    root.render(
+      <ScrollView
+        style={{height: 100, width: 100}}
+        ref={node => {
+          maybeNode = node;
+        }}>
+        <View
+          style={{
+            marginTop: 150,
+          }}>
+          <View
+            nativeID={'child'}
+            style={{height: 10, width: 10, backgroundColor: 'red'}}
+          />
+        </View>
+      </ScrollView>,
+    );
+  });
+
+  expect(root.takeMountingManagerLogs()).toEqual([
+    'Update {type: "View", nativeID: "child"}',
+    'Remove {type: "View", parentNativeID: (N/A), index: 0, nativeID: "child"}',
+    'Remove {type: "View", parentNativeID: (N/A), index: 0, nativeID: (N/A)}',
+    'Delete {type: "View", nativeID: (N/A)}',
+    'Insert {type: "View", parentNativeID: (N/A), index: 0, nativeID: "child"}',
+  ]);
+});
diff --git a/packages/react-native/ReactCommon/react/renderer/mounting/Differentiator.cpp b/packages/react-native/ReactCommon/react/renderer/mounting/Differentiator.cpp
index eef05353a07..c47af72936a 100644
--- a/packages/react-native/ReactCommon/react/renderer/mounting/Differentiator.cpp
+++ b/packages/react-native/ReactCommon/react/renderer/mounting/Differentiator.cpp
@@ -201,6 +201,11 @@ static void updateMatchedPairSubtrees(
                  << newPair << " with parent [" << parentTag << "]";
     });
 
+    auto oldCullingContextCopy =
+        oldCullingContext.adjustCullingContextIfNeeded(oldPair);
+    auto newCullingContextCopy =
+        newCullingContext.adjustCullingContextIfNeeded(newPair);
+
     // Flattening
     if (!oldPair.flattened) {
       // Flatten old tree into new list
@@ -217,8 +222,8 @@ static void updateMatchedPairSubtrees(
           oldPair.shadowView.tag,
           nullptr,
           nullptr,
-          oldCullingContext,
-          newCullingContext);
+          oldCullingContextCopy,
+          newCullingContextCopy);
     }
     // Unflattening
     else {
@@ -230,7 +235,7 @@ static void updateMatchedPairSubtrees(
       // + zIndex: the children could be listed before the parent,
       // interwoven with children from other nodes, etc.
       auto oldFlattenedNodes = sliceChildShadowNodeViewPairsFromViewNodePair(
-          oldPair, scope, true, oldCullingContext);
+          oldPair, scope, true, oldCullingContextCopy);
       for (size_t i = 0, j = 0;
            i < oldChildPairs.size() && j < oldFlattenedNodes.size();
            i++) {
@@ -252,8 +257,8 @@ static void updateMatchedPairSubtrees(
           parentTag,
           nullptr,
           nullptr,
-          oldCullingContext,
-          newCullingContext);
+          oldCullingContextCopy,
+          newCullingContextCopy);
 
       // If old nodes were not visited, we know that we can delete
       // them now. They will be removed from the hierarchy by the