diff --git a/packages/react-native/Libraries/Components/ScrollView/__tests__/ScrollView-viewCulling-itest.js b/packages/react-native/Libraries/Components/ScrollView/__tests__/ScrollView-viewCulling-itest.js
index 3c33c659e72..6e4c661b78f 100644
--- a/packages/react-native/Libraries/Components/ScrollView/__tests__/ScrollView-viewCulling-itest.js
+++ b/packages/react-native/Libraries/Components/ScrollView/__tests__/ScrollView-viewCulling-itest.js
@@ -2556,3 +2556,56 @@ describe('culling inside ScrollView with overflow visible', () => {
     );
   });
 });
+
+describe('horizontal ScrollView in RTL script', () => {
+  it('renders item 1', () => {
+    const root = Fantom.createRoot({viewportWidth: 100, viewportHeight: 100});
+
+    Fantom.runTask(() => {
+      root.render(
+        <ScrollView
+          style={{direction: 'rtl', height: 100, width: 100}}
+          horizontal={true}>
+          <View nativeID={'item1'} style={{height: 90, width: 90, margin: 5}} />
+          <View nativeID={'item2'} style={{height: 90, width: 90, margin: 5}} />
+        </ScrollView>,
+      );
+    });
+
+    expect(root.takeMountingManagerLogs()).toEqual([
+      'Update {type: "RootView", nativeID: (root)}',
+      'Create {type: "ScrollView", nativeID: (N/A)}',
+      'Create {type: "AndroidHorizontalScrollContentView", nativeID: (N/A)}',
+      'Create {type: "View", nativeID: "item1"}',
+      'Insert {type: "View", parentNativeID: (N/A), index: 0, nativeID: "item1"}',
+      'Insert {type: "AndroidHorizontalScrollContentView", parentNativeID: (N/A), index: 0, nativeID: (N/A)}',
+      'Insert {type: "ScrollView", parentNativeID: (root), index: 0, nativeID: (N/A)}',
+    ]);
+  });
+
+  it('takes contentOffset into account', () => {
+    const root = Fantom.createRoot({viewportWidth: 100, viewportHeight: 100});
+
+    Fantom.runTask(() => {
+      root.render(
+        <ScrollView
+          style={{direction: 'rtl', height: 100, width: 100}}
+          horizontal={true}
+          contentOffset={{x: 100, y: 0}}>
+          <View nativeID={'item1'} style={{height: 90, width: 90, margin: 5}} />
+          <View nativeID={'item2'} style={{height: 90, width: 90, margin: 5}} />
+        </ScrollView>,
+      );
+    });
+
+    expect(root.takeMountingManagerLogs()).toEqual([
+      'Update {type: "RootView", nativeID: (root)}',
+      'Create {type: "ScrollView", nativeID: (N/A)}',
+      'Create {type: "AndroidHorizontalScrollContentView", nativeID: (N/A)}',
+      'Create {type: "View", nativeID: "item2"}',
+      'Insert {type: "View", parentNativeID: (N/A), index: 0, nativeID: "item2"}',
+      'Insert {type: "AndroidHorizontalScrollContentView", parentNativeID: (N/A), index: 0, nativeID: (N/A)}',
+      'Insert {type: "ScrollView", parentNativeID: (root), index: 0, nativeID: (N/A)}',
+    ]);
+  });
+});
diff --git a/packages/react-native/ReactCommon/react/renderer/mounting/internal/CullingContext.cpp b/packages/react-native/ReactCommon/react/renderer/mounting/internal/CullingContext.cpp
index b061451ac0f..52c539965de 100644
--- a/packages/react-native/ReactCommon/react/renderer/mounting/internal/CullingContext.cpp
+++ b/packages/react-native/ReactCommon/react/renderer/mounting/internal/CullingContext.cpp
@@ -25,12 +25,24 @@ CullingContext CullingContext::adjustCullingContextIfNeeded(
             dynamic_cast<const ScrollViewShadowNode*>(pair.shadowNode)) {
       if (scrollViewShadowNode->getConcreteProps().yogaStyle.overflow() !=
           yoga::Overflow::Visible) {
+        auto layoutMetrics = scrollViewShadowNode->getLayoutMetrics();
         cullingContext.frame.origin =
             -scrollViewShadowNode->getContentOriginOffset(
                 /* includeTransform */ true);
         cullingContext.frame.size =
             scrollViewShadowNode->getLayoutMetrics().frame.size;
         cullingContext.transform = Transform::Identity();
+
+        if (layoutMetrics.layoutDirection == LayoutDirection::RightToLeft) {
+          // In RTL, content offset is flipped horizontally.
+          // We need to flip the culling context frame to match.
+          // See:
+          // https://github.com/facebook/react-native/blob/c2f39cfdd87c32b9a59efe8a788b8a03f02b0ea0/packages/react-native/React/Fabric/Mounting/ComponentViews/ScrollView/RCTScrollViewComponentView.mm#L579
+          auto stateData = scrollViewShadowNode->getStateData();
+          cullingContext.frame.origin.x =
+              stateData.contentBoundingRect.size.width -
+              layoutMetrics.frame.size.width - cullingContext.frame.origin.x;
+        }
       } else {
         cullingContext = {};
       }