diff --git a/packages/react-native/Libraries/ReactNative/ReactFabricPublicInstance/__tests__/ReactFabricPublicInstance-Legacy-itest.js b/packages/react-native/Libraries/ReactNative/ReactFabricPublicInstance/__tests__/ReactFabricPublicInstance-Legacy-itest.js
index 6c1477aa7b5..1970056a68f 100644
--- a/packages/react-native/Libraries/ReactNative/ReactFabricPublicInstance/__tests__/ReactFabricPublicInstance-Legacy-itest.js
+++ b/packages/react-native/Libraries/ReactNative/ReactFabricPublicInstance/__tests__/ReactFabricPublicInstance-Legacy-itest.js
@@ -7,6 +7,7 @@
  * @flow strict-local
  * @format
  * @oncall react_native
+ * @fantom_flags enableAccessToHostTreeInFabric:false
  */
 
 import setUpReactFabricPublicInstanceFantomTests from './setUpReactFabricPublicInstanceFantomTests';
diff --git a/packages/react-native/Libraries/ReactNative/ReactFabricPublicInstance/__tests__/ReactFabricPublicInstance-Modern-itest.js b/packages/react-native/Libraries/ReactNative/ReactFabricPublicInstance/__tests__/ReactFabricPublicInstance-Modern-itest.js
index 9f66d43eacc..f6ca539c85b 100644
--- a/packages/react-native/Libraries/ReactNative/ReactFabricPublicInstance/__tests__/ReactFabricPublicInstance-Modern-itest.js
+++ b/packages/react-native/Libraries/ReactNative/ReactFabricPublicInstance/__tests__/ReactFabricPublicInstance-Modern-itest.js
@@ -7,9 +7,9 @@
  * @flow strict-local
  * @format
  * @oncall react_native
+ * @fantom_flags enableAccessToHostTreeInFabric:true
  */
 
-import './setUpFeatureFlags';
 import setUpReactFabricPublicInstanceFantomTests from './setUpReactFabricPublicInstanceFantomTests';
 
 setUpReactFabricPublicInstanceFantomTests({isModern: true});
diff --git a/packages/react-native/Libraries/ReactNative/ReactFabricPublicInstance/__tests__/setUpFeatureFlags.js b/packages/react-native/Libraries/ReactNative/ReactFabricPublicInstance/__tests__/setUpFeatureFlags.js
deleted file mode 100644
index a5a75c44ac4..00000000000
--- a/packages/react-native/Libraries/ReactNative/ReactFabricPublicInstance/__tests__/setUpFeatureFlags.js
+++ /dev/null
@@ -1,16 +0,0 @@
-/**
- * Copyright (c) Meta Platforms, Inc. and affiliates.
- *
- * This source code is licensed under the MIT license found in the
- * LICENSE file in the root directory of this source tree.
- *
- * @flow strict-local
- * @format
- * @oncall react_native
- */
-
-import * as ReactNativeFeatureFlags from '../../../../src/private/featureflags/ReactNativeFeatureFlags';
-
-ReactNativeFeatureFlags.override({
-  enableAccessToHostTreeInFabric: () => true,
-});
diff --git a/packages/react-native/src/private/webapis/intersectionobserver/__tests__/IntersectionObserver-itest.js b/packages/react-native/src/private/webapis/intersectionobserver/__tests__/IntersectionObserver-itest.js
index f034867e8fa..e6687af4c8e 100644
--- a/packages/react-native/src/private/webapis/intersectionobserver/__tests__/IntersectionObserver-itest.js
+++ b/packages/react-native/src/private/webapis/intersectionobserver/__tests__/IntersectionObserver-itest.js
@@ -7,6 +7,7 @@
  * @flow strict-local
  * @format
  * @oncall react_native
+ * @fantom_flags enableAccessToHostTreeInFabric:true
  */
 
 /* eslint-disable lint/sort-imports */
@@ -19,7 +20,6 @@ import ReactNativeElement from '../../dom/nodes/ReactNativeElement';
 import IntersectionObserverEntry from '../IntersectionObserverEntry';
 import * as React from 'react';
 
-import './setUpFeatureFlags';
 import '../../../../../Libraries/Core/InitializeCore.js';
 
 import ScrollView from '../../../../../Libraries/Components/ScrollView/ScrollView';
diff --git a/packages/react-native/src/private/webapis/intersectionobserver/__tests__/setUpFeatureFlags.js b/packages/react-native/src/private/webapis/intersectionobserver/__tests__/setUpFeatureFlags.js
deleted file mode 100644
index 5af0f6f71dd..00000000000
--- a/packages/react-native/src/private/webapis/intersectionobserver/__tests__/setUpFeatureFlags.js
+++ /dev/null
@@ -1,16 +0,0 @@
-/**
- * Copyright (c) Meta Platforms, Inc. and affiliates.
- *
- * This source code is licensed under the MIT license found in the
- * LICENSE file in the root directory of this source tree.
- *
- * @flow strict-local
- * @format
- * @oncall react_native
- */
-
-import * as ReactNativeFeatureFlags from '../../../featureflags/ReactNativeFeatureFlags';
-
-ReactNativeFeatureFlags.override({
-  enableAccessToHostTreeInFabric: () => true,
-});
diff --git a/packages/react-native/src/private/webapis/mutationobserver/__tests__/MutationObserver-itest.js b/packages/react-native/src/private/webapis/mutationobserver/__tests__/MutationObserver-itest.js
index d14ec74a2dc..695ea2c608f 100644
--- a/packages/react-native/src/private/webapis/mutationobserver/__tests__/MutationObserver-itest.js
+++ b/packages/react-native/src/private/webapis/mutationobserver/__tests__/MutationObserver-itest.js
@@ -7,6 +7,7 @@
  * @flow strict-local
  * @format
  * @oncall react_native
+ * @fantom_flags enableAccessToHostTreeInFabric:true
  */
 
 import type MutationObserverType from '../MutationObserver';
@@ -18,7 +19,6 @@ import ReactNativeElement from '../../dom/nodes/ReactNativeElement';
 import nullthrows from 'nullthrows';
 import * as React from 'react';
 
-import './setUpFeatureFlags';
 import '../../../../../Libraries/Core/InitializeCore.js';
 
 declare const MutationObserver: Class<MutationObserverType>;
diff --git a/packages/react-native/src/private/webapis/mutationobserver/__tests__/setUpFeatureFlags.js b/packages/react-native/src/private/webapis/mutationobserver/__tests__/setUpFeatureFlags.js
deleted file mode 100644
index 5af0f6f71dd..00000000000
--- a/packages/react-native/src/private/webapis/mutationobserver/__tests__/setUpFeatureFlags.js
+++ /dev/null
@@ -1,16 +0,0 @@
-/**
- * Copyright (c) Meta Platforms, Inc. and affiliates.
- *
- * This source code is licensed under the MIT license found in the
- * LICENSE file in the root directory of this source tree.
- *
- * @flow strict-local
- * @format
- * @oncall react_native
- */
-
-import * as ReactNativeFeatureFlags from '../../../featureflags/ReactNativeFeatureFlags';
-
-ReactNativeFeatureFlags.override({
-  enableAccessToHostTreeInFabric: () => true,
-});