diff --git a/packages/react-native/src/private/setup/__tests__/setUpDefaultReactNativeEnvironment-FeatureFlags-itest.js b/packages/react-native/src/private/setup/__tests__/setUpDefaultReactNativeEnvironment-FeatureFlags-itest.js
new file mode 100644
index 00000000000..b6f7acd4be4
--- /dev/null
+++ b/packages/react-native/src/private/setup/__tests__/setUpDefaultReactNativeEnvironment-FeatureFlags-itest.js
@@ -0,0 +1,28 @@
+/**
+ * Copyright (c) Meta Platforms, Inc. and affiliates.
+ *
+ * This source code is licensed under the MIT license found in the
+ * LICENSE file in the root directory of this source tree.
+ *
+ * @flow strict-local
+ * @format
+ */
+
+import * as ReactNativeFeatureFlags from '../../featureflags/ReactNativeFeatureFlags';
+import * as ReactNativeFeatureFlagsBase from '../../featureflags/ReactNativeFeatureFlagsBase';
+import setUpDefaultReactNativeEnvironment from 'react-native/src/private/setup/setUpDefaultReactNativeEnvironment';
+
+describe('setUpReactNativeEnvironment', () => {
+  it('should not read any feature flags', () => {
+    ReactNativeFeatureFlagsBase.dangerouslyResetForTesting();
+
+    setUpDefaultReactNativeEnvironment(false);
+
+    expect(() => {
+      // If any feature flags were read, this call would fail.
+      ReactNativeFeatureFlags.override({
+        jsOnlyTestFlag: () => true,
+      });
+    }).not.toThrow();
+  });
+});