diff --git a/packages/react-native/React/Fabric/Mounting/ComponentViews/ScrollView/RCTScrollViewComponentView.mm b/packages/react-native/React/Fabric/Mounting/ComponentViews/ScrollView/RCTScrollViewComponentView.mm
index 5abc1682ed1..a3191aa4372 100644
--- a/packages/react-native/React/Fabric/Mounting/ComponentViews/ScrollView/RCTScrollViewComponentView.mm
+++ b/packages/react-native/React/Fabric/Mounting/ComponentViews/ScrollView/RCTScrollViewComponentView.mm
@@ -112,6 +112,11 @@ RCTSendScrollEventForNativeAnimations_DEPRECATED(UIScrollView *scrollView, NSInt
   __weak UIView *_firstVisibleView;
 
   CGFloat _endDraggingSensitivityMultiplier;
+
+  // A flag indicating that accessibility API is used.
+  // It is not restored to the default value in prepareForRecycle.
+  // Once an accessibility API is used, view culling will be disabled for the entire session.
+  BOOL _isAccessibilityAPIUsed;
 }
 
 + (RCTScrollViewComponentView *_Nullable)findScrollViewComponentViewForView:(UIView *)view
@@ -134,6 +139,7 @@ RCTSendScrollEventForNativeAnimations_DEPRECATED(UIScrollView *scrollView, NSInt
     _isUserTriggeredScrolling = NO;
     _shouldUpdateContentInsetAdjustmentBehavior = YES;
     _automaticallyAdjustKeyboardInsets = NO;
+    _isAccessibilityAPIUsed = NO;
     [self addSubview:_scrollView];
 
     _containerView = [[UIView alloc] initWithFrame:CGRectZero];
@@ -593,6 +599,38 @@ static inline UIViewAnimationOptions animationOptionsWithCurve(UIViewAnimationCu
   return metrics;
 }
 
+/**
+ * When use of accessibility APIs is detected, view culling is disabled to make accessibility work correctly.
+ */
+- (void)_disableViewCullingIfNecessary
+{
+  if (!_isAccessibilityAPIUsed) {
+    _isAccessibilityAPIUsed = YES;
+    [self _updateStateWithContentOffset];
+  }
+}
+
+- (NSInteger)accessibilityElementCount
+{
+  // From empirical testing, method `accessibilityElementCount` is called lazily only
+  // when accessibility is used.
+  // Why we don't use UIAccessibilitySwitchControlStatusDidChangeNotification and
+  // UIAccessibilityVoiceOverStatusDidChangeNotification? The notifications are not called when using Accessibility
+  // Inspector. We anticipate developers will want to debug accessbility with Accessibility Inspector and don't want to
+  // break that developer workflow with view culling. Therefore, we are using API use detection to disable view culling
+  // instead of the notifications.
+  [self _disableViewCullingIfNecessary];
+  return [super accessibilityElementCount];
+}
+
+- (NSArray<id<UIFocusItem>> *)focusItemsInRect:(CGRect)rect
+{
+  // From empirical testing, method `focusItemsInRect:` is called lazily only
+  // when keyboard navigation is used.
+  [self _disableViewCullingIfNecessary];
+  return [super focusItemsInRect:rect];
+}
+
 - (void)_updateStateWithContentOffset
 {
   if (!_state) {
@@ -600,15 +638,18 @@ static inline UIViewAnimationOptions animationOptionsWithCurve(UIViewAnimationCu
   }
 
   auto contentOffset = RCTPointFromCGPoint(_scrollView.contentOffset);
+  BOOL isAccessibilityAPIUsed = _isAccessibilityAPIUsed;
   _state->updateState(
-      [contentOffset](
+      [contentOffset, isAccessibilityAPIUsed](
           const ScrollViewShadowNode::ConcreteState::Data &oldData) -> ScrollViewShadowNode::ConcreteState::SharedData {
-        if (oldData.contentOffset == contentOffset) {
-          // avoid doing a state update if content offset didn't change.
+        if (oldData.contentOffset == contentOffset && oldData.disableViewCulling == isAccessibilityAPIUsed) {
+          // avoid doing a state update if content offset and use of accessibility didn't change.
           return nullptr;
         }
         auto newData = oldData;
         newData.contentOffset = contentOffset;
+        newData.disableViewCulling =
+            UIAccessibilityIsVoiceOverRunning() || UIAccessibilityIsSwitchControlRunning() || isAccessibilityAPIUsed;
         return std::make_shared<const ScrollViewShadowNode::ConcreteState::Data>(newData);
       },
       ReactNativeFeatureFlags::enableImmediateUpdateModeForContentOffsetChanges()
diff --git a/packages/react-native/ReactCommon/react/renderer/components/scrollview/ScrollViewState.h b/packages/react-native/ReactCommon/react/renderer/components/scrollview/ScrollViewState.h
index bfe5d7f308f..e4a53e61704 100644
--- a/packages/react-native/ReactCommon/react/renderer/components/scrollview/ScrollViewState.h
+++ b/packages/react-native/ReactCommon/react/renderer/components/scrollview/ScrollViewState.h
@@ -33,6 +33,14 @@ class ScrollViewState final {
   Rect contentBoundingRect;
   int scrollAwayPaddingTop;
 
+  /*
+   * View Culling has to be disabled when accessibility features are used.
+   * View Culling removes views from view hierarchy and for example VoiceOver
+   * wouldn't recognise there is a view outside of the viewport that it can
+   * scroll to.
+   */
+  bool disableViewCulling{false};
+
   /*
    * Returns size of scrollable area.
    */
diff --git a/packages/react-native/ReactCommon/react/renderer/mounting/internal/CullingContext.cpp b/packages/react-native/ReactCommon/react/renderer/mounting/internal/CullingContext.cpp
index 52c539965de..8294edb254b 100644
--- a/packages/react-native/ReactCommon/react/renderer/mounting/internal/CullingContext.cpp
+++ b/packages/react-native/ReactCommon/react/renderer/mounting/internal/CullingContext.cpp
@@ -24,7 +24,8 @@ CullingContext CullingContext::adjustCullingContextIfNeeded(
     if (auto scrollViewShadowNode =
             dynamic_cast<const ScrollViewShadowNode*>(pair.shadowNode)) {
       if (scrollViewShadowNode->getConcreteProps().yogaStyle.overflow() !=
-          yoga::Overflow::Visible) {
+              yoga::Overflow::Visible &&
+          !scrollViewShadowNode->getStateData().disableViewCulling) {
         auto layoutMetrics = scrollViewShadowNode->getLayoutMetrics();
         cullingContext.frame.origin =
             -scrollViewShadowNode->getContentOriginOffset(